%0 Research article
%T Effects of Melampyrum extracts on the growth of axenic cultures of Cronartium flaccidum and Peridermium pini
%A Kaitera, Juha
%A Nuorteva, Heikki
%D 2010
%J Silva Fennica
%V 44
%N 2
%R doi:10.14214/sf.149
%U https://silvafennica.fi/article/149
%X For 3–6 months, mycelial colonies cultured from 5 isolates of each of  two pine stem rusts (Cronartium flaccidum and Peridermium pini) were  grown on nutrient-rich agar supplemented with Melampyrum extracts.  Non-autoclaved extracts of M. pratense significantly reduced the growth  of P. pini. The growth of C. flaccidum isolates was slightly stimulated  after the second month of incubation but after that was inhibited during  incubation months 4–6. We observed considerable variation in colony  growth, a significant component of which was explained by incubation  time, isolate, growth medium and their interaction. Rust species (C.  flaccidum or P. pini) was not an important factor in growth variation.  While sterilized extracts of M. pratense, M. sylvaticum and M. nemorosum  did not significantly affect growth, colonies of C. flaccidum were  slightly stimulated, whereas colonies of P. pini were slightly  inhibited. Generally, isolates of P. pini grew better and showed a  slower rate of degeneration than C. flaccidum on all media.