Information on forest biomass is required for several purposes, including estimation of forest bioenergy resources and forest carbon stocks. Airborne laser scanning is today considered the most accurate remote sensing method for forest inventory. The three-dimensional nature of laser scanning data enables estimation of the volumes of the tree canopies. The dimensions of the tree canopies show high correlation with the amount of forest biomass. Optical aerial photographs are often used to complement laser data, for improved distinction between tree species. The paper reports on a study testing the estimation of forest biomass variables in two study areas in Southern Finland. The biomass variables were derived on the basis of tree-level field measurements, with biomass models used for pine, spruce, and birch. The sample-plot-level biomass components were derived on the basis of tree-level data and used as reference data for airborne-laser- and aerial‑photograph-based estimation. Results were slightly better for total biomass (RMSE 22.5% and 23.6% for the two study areas) than total volume (RMSE: 23.4% and 26.1%). Species-specific estimation errors were large in general but varied between the study areas, because of differences in their forest structures.