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Articles containing the keyword 'NIRS'.

Category: Research article

article id 9996, category Research article
Mulualem Tigabu, Mostafa Farhadi, Lars-Göran Stener, Per C. Odén. (2018). Visible + Near Infrared Spectroscopy as taxonomic tool for identifying birch species. Silva Fennica vol. 52 no. 4 article id 9996. https://doi.org/10.14214/sf.9996
Highlights: Multivariate modelling of visible + near infrared (NIR) reflectance spectra of single seeds distinguished Betula pubescens and B. pendula with 100% and 99% accuracy, respectively; The results demonstrate the feasibility of NIR spectroscopy as taxonomic tool for classification of species that have morphological resemblance.

The genus Betula L. is composed of several species, which are difficult to distinguish in the field on the basis of morphological traits. The aim of this study was to evaluate the taxonomic importance of using visible + near infrared (Vis + NIR) spectra of single seeds for differentiating Betula pendula Roth and Betula pubescens Ehrh. Seeds from several families (controlled crossings of known parent trees) of each species were used and Vis + NIR reflectance spectra were obtained from single seeds. Multivariate discriminant models were developed by Orthogonal Projections to Latent Structures – Discriminant Analysis (OPLS-DA). The OPLS-DA model fitted on Vis + NIR spectra recognized B. pubescens with 100% classification accuracy while the prediction accuracy of class membership for B. pendula was 99%. However, the discriminant models fitted on NIR spectra alone resulted in 100% classification accuracies for both species. Absorption bands accounted for distinguishing between birch species were attributed to differences in color and chemical composition, presumably polysaccharides, proteins and fatty acids, of the seeds. In conclusion, the results demonstrate the feasibility of NIR spectroscopy as taxonomic tool for classification of species that have morphological resemblance.

  • Tigabu, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, Box 49, SE-230 52 Alnarp, Sweden ORCID ID:E-mail: mulualem.tigabu@slu.se (email)
  • Farhadi, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, Box 49, SE-230 52 Alnarp, Sweden ORCID ID:E-mail: mostafa.farhadi@gmail.com
  • Stener, The Forestry Research Institute of Sweden, Ekebo 2250, SE-268 90 Svalöv, Sweden ORCID ID:E-mail: lars-goran.stener@skogforsk.se
  • Odén, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, Box 49, SE-230 52 Alnarp, Sweden ORCID ID:E-mail: per.christer.oden@slu.se
article id 7822, category Research article
Mulualem Tigabu, Annika M. Felton. (2018). Multivariate calibration of near infrared spectra for predicting nutrient concentrations of solid moose rumen contents. Silva Fennica vol. 52 no. 1 article id 7822. https://doi.org/10.14214/sf.7822
Highlights: Multivariate calibrations were established for predicting nutrient concentrations of solid moose rumen contents by near infrared spectroscopy (NIRS); Crude protein, available protein and ash contents were accurately predicted; Prediction of microbial nitrogen, ash, acid-detergent fiber, neutral detergent fiber and lignin were satisfactory; The results demonstrate that NIRS offers quick and inexpensive procedure to quantify nutrient concentrations of solid rumen contents.

This study aimed at establishing calibrations to predict nutrient concentrations of solid moose (Alces alces L.) rumen content using near infrared spectroscopy (NIRS), as an alternative to expensive chemical analyses. NIR reflectance spectra of 148 dry pulverized samples were recorded. The scanned samples were then analyzed for crude protein, available protein, microbial nitrogen (N), ash, acid-detergent fiber (ADF), neutral detergent fiber (NDF) and lignin contents following standard chemical analysis procedures. The calibration models were derived by Orthogonal Projection to Latent Structure (OPLS) and validated using external prediction sets. The calibration models accurately predicted crude protein, available protein and ash contents (R2 = 0.99, 0.96, and 0.92, prediction error = 0.39, 0.72 and 0.53% dry matter, respectively) while NDF (R2 = 0.92; prediction error = 2.23% dry matter) and ADF (R2 = 0.89; prediction error = 1.94% dry matter) were predicted with sufficient accuracy and that of microbial-N (R2 = 0.81; prediction error = 1.25 mg yeast-RNA g–1 dry matter) and lignin (R2 = 0.84; prediction error = 1.05% dry matter) were acceptable. The ratio of performance to deviation values were > 3.0 for crude protein and available protein, between 3.0 and 2.5 for ADF, NDF and lignin, and 2.32 for microbial-N; attesting the robustness of the calibration models. It can be concluded that NIR spectroscopy offers a quick and inexpensive procedure for prediction of nutrient concentrations of solid rumen contents in wild herbivores.

  • Tigabu, Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, Box 49, SE-230 53 Alnarp, Sweden ORCID ID: http://orcid.org/0000-0003-2471-1168 E-mail: mulualem.tigabu@slu.se (email)
  • Felton, Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, Box 49, SE-230 53 Alnarp, Sweden ORCID ID:E-mail: annika.felton@slu.se
article id 1340, category Research article
Mostafa Farhadi, Mulualem Tigabu, Per Christer Odén. (2015). Near Infrared Spectroscopy as non-destructive method for sorting viable, petrified and empty seeds of Larix sibirica. Silva Fennica vol. 49 no. 5 article id 1340. https://doi.org/10.14214/sf.1340
Highlights: Near Infrared spectroscopy discriminates filled-viable, empty and petrified seeds of Larix sibirica with 98%, 82% and 87% accuracy, respectively based on spectral differences attributed to moisture and storage reserves; The classification accuracy reached 100% when sorting seeds into viable and non-viable class; The results demonstrate that NIR spectroscopy has great potential as non-destructive sorting technique to upgrade seed lot quality.

Larix sibirica Ledeb. is one of the promising timber species for planting in the boreal ecosystem; but poor seed lot quality is the major hurdle for production of sufficient quantity of planting stocks. Here, we evaluated the potential of Near Infrared (NIR) Spectroscopy for sorting viable and non-viable seeds, as the conventional sorting technique is inefficient. NIR reflectance spectra were collected from single seeds, and discriminant models were developed with Orthogonal Projections to Latent Structure – Discriminant Analysis (OPLS-DA). The computed model predicted the class membership of filled-viable, empty and petrified seeds in the test set with 98%, 82% and 87% accuracy, respectively. When two-class OPLS-DA model was fitted to discriminate viable and non-viable (empty and petrified seeds combined), the predicted class membership of test set samples was 100% for both classes. The origins of spectral differences between non-viable (petrified and empty) and viable seeds were attributed to differences in seed moisture content and storage reserves. In conclusion, the result provides evidence that NIR spectroscopy is a powerful non-destructive method for sorting non-viable seeds of Larix sibirica; thus efforts should be made to develop on-line sorting system for large-scale seed handling.

  • Farhadi, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53 Alnarp, Sweden ORCID ID:E-mail: mostafa.farhadi@slu.se
  • Tigabu, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53 Alnarp, Sweden ORCID ID:E-mail: mulualem.tigabu@slu.se (email)
  • Odén, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53 Alnarp, Sweden ORCID ID:E-mail: per.oden@slu.se
article id 1334, category Research article
Abolfazl Daneshvar, Mulualem Tigabu, Asaddollah Karimidoost, Per Christer Oden. (2015). Single seed Near Infrared Spectroscopy discriminates viable and non-viable seeds of Juniperus polycarpos. Silva Fennica vol. 49 no. 5 article id 1334. https://doi.org/10.14214/sf.1334
Highlights: Near Infrared (NIR) Spectroscopy discriminates viable and non-viable (empty, insect-attacked and shriveled) seeds of J. polycarpos with 98% and 100% accuracy, respectively; The origins of spectral differences between non-viable and viable seeds were attributed to differences in seed coat chemical composition and storage reserves; The results demonstrate that NIR spectroscopy has great potential as seed sorting technology to ensure precision sowing.

A large quantity of non-viable (empty, insect-attacked and shriveled) seeds of Juniperus polycarpos (K. Koch) is often encountered during seed collection, which should be removed from the seed lots to ensure precision sowing in the nursery or out in the field. The aims of this study were to evaluate different modelling approaches and to examine the sensitivity of the change in detection system (Silicon-detector in the shorter vis-a-vis InGsAs-detector in the longer NIR regions) for discriminating non-viable seeds from viable seeds by Near Infrared (NIR) spectroscopy. NIR reflectance spectra were collected from single seeds, and discriminant models were developed by Partial Least Squares – Discriminant Analysis (PLS-DA) and Orthogonal Projection to Latent Structures – Discriminant Analysis (OPLS-DA) using the entire or selected NIR regions. Both modelling approaches resulted in 98% and 100% classification accuracy for viable and non-viable seeds in the test set, respectively. However, OPLS-DA models were superb in terms of model parsimony and information quality. Modelling in the shorter and longer wavelength region also resulted in similar classification accuracy, suggesting that prediction of class membership is insensitive to change in the detection system. The origins of spectral differences between non-viable and viable seeds were attributed to differences in seed coat chemical composition, mainly terpenoids that were dominant in non-viable seeds and storage reserves in viable seeds. In conclusion, the results demonstrate that NIR spectroscopy has great potential as seed sorting technology to upgrade seed lot quality that ensures precision sowing.

  • Daneshvar, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53, Alnarp, Sweden; (permanent address) Department of Natural Resources, Gonbad Kavous University, Shahid Fallahi Street, P.O. Box 163, Gonbad, Iran ORCID ID:E-mail: abolfazl.daneshvar@slu.se
  • Tigabu, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53, Alnarp, Sweden ORCID ID:E-mail: mulualem.tigabu@slu.se (email)
  • Karimidoost, Agriculture and Natural Resources Research Center of Golestan Province, Beheshti Ave. P.O. Box 4915677555, Gorgan, Iran ORCID ID:E-mail: karimidoost@yahoo.com
  • Oden, Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53, Alnarp, Sweden ORCID ID:E-mail: per.oden@slu.se

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