Table 1. Characteristics of four studied populations of Prunus serotina located in Pinus sylvestris stands.
Population ID Location Number of individuals sampled Habitat
type 		Invasion
stage 		No of adults Density
(indv./m2) 		Age P. sylvestris stand age
Raszków R 51°42’37.89” N
17°44’32.12” E 		109 agricultural colonization (isolated stand) 8 0.18 17 34
Zielonka Forest Z_1 52°30’26.17” N
17°07’08.52” E 		180 forest colonization 3 0.68 20 34
Zielonka Forest Z_2 52°31’00.81” N
17°06’44.46” E 		446 forest established 16 1.82 35 80
Zielonka Forest Z_3 52°31’01.44” N
17°06’57.01” E 		205 forest established 16 0.84 35 146
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Fig. 1. Sampling scheme in study plots located in four analyzed populations of Prunus serotina. Open circles define location of mature (fruiting) trees. View larger in new window/tab

Table 2. Characteristics of eight microsatellite loci used.
Locus A HO HE FIS Null
UCD_CH14 17 0.761 0.782 0.027 0.023
UDP96_005 6 0.482 0.584 0.175 0.070
UDP98_025 9 0.723 0.757 0.045 0.027
PCGA43 18 0.738 0.758 0.026 0.020
PCHGMS3_1 20 0.639 0.813 0.214 0.090
PCHGMS3_2 21 0.708 0.820 0.137 0.056
PCHGSM2 18 0.642 0.674 0.047 0.022
UDP98-405 12 0.474 0.564 0.161 0.062
Average 15.1 0.646 0.719 0.104 0.046
A – number of alleles, HO – observed heterozygosity, HE – expected heterozygosity, FIS – inbreeding coefficient, Null – frequency of null alleles
Table 3. Results of genetic structure, clonal diversity and pedigree analysis conducted in four populations of Prunus serotina. View in new window/tab.
Table 4. Fine-scale genetic structure parameters for each population.
Studied populations Fij bF Sp
R Genets Ramets 0.0346 (0.0075) 0.1568 (0.0239) –0.01402*** (0.0033) –0.05107*** (0.0089) 0.0145 0.0601
Z_1 Genets Ramets 0.0143 (0.0030) 0.0195 (0.0040) –0.00672*** (0.0011) –0.01010*** (0.0017) 0.0068 0.0103
Z_2 Genets Ramets 0.0119 (0.0011) 0.0120 (0.0011) –0.00542*** (0.0006) –0.00558*** (0.0006) 0.0054 0.0056
Z_3 Genets Ramets 0.0232 (0.0059) 0.0258 (0.0057) –0.00648*** (0.0017) –0.00515*** (0.0012) 0.0066 0.0053
Fij – the mean kinship coefficient between individuals in the first distance class (5 m), bF – slope of the regression of kinship coefficients on the logarithm of spatial distance, Sp – intensity of FSGS, *** – significant at 0.001, SE – in brackets.
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Fig. 2. Spatial correlograms for four analyzed Prunus serotina populations with mean pairwise kinship coefficients (Fij) of six distance classes for eight microsatellite loci. Upper and lower 95% confidence intervals for null hypothesis of random distribution of individuals are shaded. View larger in new window/tab