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Matti Huotari (email), Mareena Jaskari, Erkki Annila, Vilho Lantto

Responses of olfactory receptor neurons of the large pine weevil to a possible deterrent Neutroil® and two other chemicals

Huotari M., Jaskari M., Annila E., Lantto V. (2003). Responses of olfactory receptor neurons of the large pine weevil to a possible deterrent Neutroil® and two other chemicals. Silva Fennica vol. 37 no. 1 article id 517. https://doi.org/10.14214/sf.517

Abstract

Electrophysiological responses of olfactory receptor neurons were measured from the antennal sensilla of large pine weevils (Hylobius abietis L.) at 1 s exposure to Neutroil®-hexane mixture odour as a possible deterrent chemical and, for comparison, to α-pinene, α-pinene-ethanol mixture, and ethanol odours, respectively. Neutroil® is a commercial chemical pulp-mill product which has been studied earlier as a deterrent for large pine weevils with preliminary feed tests. In addition, ethanol, hexane and clean carrier air responses were measured. Odour pulses and clean air, as a zero reference value, were directed at a fixed insect antenna in order to induce olfactory responses. Simultaneous olfactory responses, i.e. Hylobius electroantennograms (EAG) and action potential responses, were recorded and these responses of Hylobius olfactory receptor neurons (ORN), such as action potential rates, silent periods and EAG responses, were analyzed for all simultaneous recordings. The exposures to α-pinene, α-pinene-ethanol mixture, pure ethanol and hexane caused an increase of the action potential rate (up to 70 pulses per second) in the ORNs sensitive to these odours, while the Neutroil®-hexane mixture exposures caused either silent periods with a duration between 0.6 and 1.1 s for 1 s exposure pulses or they had no response at all in the ORNs sensitive to the other odours. Thus, the possible deterrence may be caused by inhibition of some pinene-alcohol ORNs.

Keywords
olfactory receptor neuron; attractant; repellent; Hylobius abietis; action potential; silent period; electroantennogram

Author Info
  • Huotari, University of Oulu, P.O. Box 4500, FIN-90014 University of Oulu, Finland E-mail matti.huotari@oulu.fi (email)
  • Jaskari, University of Oulu, P.O. Box 4500, FIN-90014 University of Oulu, Finland E-mail mj@nn.fi
  • Annila, Finnish Forest Research Institute, P.O. Box 18, FIN-01301 Vantaa, Finland E-mail ea@nn.fi
  • Lantto, University of Oulu, P.O. Box 4500, FIN-90014 University of Oulu, Finland E-mail vl@nn.fi

Received 5 July 2002 Accepted 12 December 2002 Published 31 December 2003

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Available at https://doi.org/10.14214/sf.517 | Download PDF

Creative Commons License CC BY-SA 4.0

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