Full text of this article is only available in PDF format.

M. Carmen San José (email), Lourdes Romero, Laura V. Janeiro

Effect of indole-3-butyric acid on root formation in Alnus glutinosa microcuttings

San José M. C., Romero L., Janeiro L. V. (2012). Effect of indole-3-butyric acid on root formation in Alnus glutinosa microcuttings. Silva Fennica vol. 46 no. 5 article id 916. https://doi.org/10.14214/sf.916

Abstract

A study of the in vitro rooting process in mature alder (Alnus glutinosa (L.) Gaertn.) shoots is described. Microcuttings from shoots cultured in vitro were transferred to a half-strength Woody Plant Medium containing 0 or 0.1 mg l–1 indole-3-butyric acid (IBA) for 0 to 7 days. The presence of IBA in the medium increased the rooting percentage, number of roots, percentage of lateral roots, and length of the shoots. Histological studies were carried out with shoots treated with 0 or 0.1 mg l–1 IBA for 7 days. According to these criteria, treatment with IBA for 2–3 days proved to be the most successful. In both treatments, substancial reactivation of cell division was observed at the base of the shoots after 1 day. Some cambial zone and adjacent phloem cells became dense cytoplasm, having nuclei with prominent nucleoli. The first cell divisions were also observed at this time. In the treatment with IBA (0.1 mg l–1 for 7 days), meristemoids became individualized, consisting of densely staining cells, by day 3. Identifiable conical shaped root primordia with several cell layers were visible after 4–5 days. Roots with an organized tissue system emerged from the stem after 6 days in the IBA-treated shoots. Meristemoid formation was delayed until the fourth day and root emergence until the eight day in the control treatment (no IBA).

Keywords
alder; mature trees; histology; rooting

Author Info
  • San José, Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda de Vigo s/n, Apartado 122, 15780 Santiago de Compostela, Spain E-mail sanjose@iiag.csic.es (email)
  • Romero, CIFP Politécnico de Santiago, Santiago de Compostela, Spain E-mail lr@nn.es
  • Janeiro, INLUDES, Diputación Provincial de Lugo, Lugo, Spain E-mail lauravj68@hotmail.com

Received 28 June 2012 Accepted 9 October 2012 Published 31 December 2012

Views 4739

Available at https://doi.org/10.14214/sf.916 | Download PDF

Creative Commons License CC BY-SA 4.0

Register
Click this link to register to Silva Fennica.
Log in
If you are a registered user, log in to save your selected articles for later access.
Contents alert
Sign up to receive alerts of new content

Your selected articles
Send to email
Ilvessalo Y., (1929) Notes on some forest (site) types in North America Acta Forestalia Fennica vol. 34 no. 39 article id 7252 (remove) | Edit comment
Kiljunen K., (1986) Growth of third world forest industry: possible .. Silva Fennica vol. 20 no. 3 article id 5271 (remove) | Edit comment
Zhang S., Korhonen L. et al. (2024) How to implement the data collection of leaf are.. Silva Fennica vol. 58 no. 5 article id 24044 (remove) | Edit comment
Morgenstern E. K., Park Y. S. (1991) Breeding of Picea mariana (Mill.) B.S.P.: seed o.. Silva Fennica vol. 25 no. 4 article id 5467 (remove) | Edit comment
Cajander A. K., (1923) On the relation between forest growth and timber.. Acta Forestalia Fennica vol. 25 no. 2 article id 7077 (remove) | Edit comment
Partanen J., Leinonen I. et al. (2001) Effect of accumulated duration of the light peri.. Silva Fennica vol. 35 no. 1 article id 608 (remove) | Edit comment
Ilvessalo Y., (1920) The relation of the productive capacity of fores.. Acta Forestalia Fennica vol. 15 no. 2 article id 7040 (remove) | Edit comment
Bergh J., Nilsson U. et al. (2008) Effects of frequency of fertilisation on product.. Silva Fennica vol. 42 no. 5 article id 225 (remove) | Edit comment
Quine C. P., Humphrey J. W. et al. (2002) An approach to predicting the potential forest c.. Silva Fennica vol. 36 no. 1 article id 560 (remove) | Edit comment
Monteleone I., Ferrazzini D. et al. (2006) Effectiveness of neutral RAPD markers to detect .. Silva Fennica vol. 40 no. 3 article id 476 (remove) | Edit comment
Pakkala T., Hanski I. et al. (2002) Spatial ecology of the three-toed woodpecker in .. Silva Fennica vol. 36 no. 1 article id 563 (remove) | Edit comment
Kärkkäinen M., (1986) Model of knottiness of wood material in pine, sp.. Silva Fennica vol. 20 no. 2 article id 5266 (remove) | Edit comment
Keltikangas M., Seppälä K. (1977) The economics of growing downy birch stands on d.. Silva Fennica vol. 11 no. 1 article id 4962 (remove) | Edit comment
Kuusipalo J., (1983) Distribution of vegetation on mesic forest sites.. Silva Fennica vol. 17 no. 4 article id 5201 (remove) | Edit comment
Yli-Vakkuri P., Autio A. et al. (1968) Time lag between final cutting and regeneration Silva Fennica vol. 2 no. 3 article id 4769 (remove) | Edit comment
Hildén N. A., (1929) Common alder forest in Kontusaari Acta Forestalia Fennica vol. 34 no. 27 article id 7240 (remove) | Edit comment
Matulevičiūtė D., Motiejunaite J. et al. (2018) Decline of a protected coastal pine forest under.. Silva Fennica vol. 52 no. 2 article id 7699 (remove) | Edit comment
Nevalainen S., (2017) Comparison of damage risks in even- and uneven-a.. Silva Fennica vol. 51 no. 3 article id 1741 (remove) | Edit comment
Norrlin J. P., (1923) Overview of moos and lichen in Tornio-Muonio and.. Acta Forestalia Fennica vol. 23 no. 6 article id 7105 (remove) | Edit comment
Víquez E., Pérez D. (2005) Effect of pruning on tree growth, yield, and woo.. Silva Fennica vol. 39 no. 3 article id 375 (remove) | Edit comment
Vohland M., Stoffels J. et al. (2007) Remote sensing techniques for forest parameter a.. Silva Fennica vol. 41 no. 3 article id 471 (remove) | Edit comment
Sirén G., (1950) On the biology of undergrown Norway spruce Acta Forestalia Fennica vol. 58 no. 2 article id 7402 (remove) | Edit comment
Leikola M., (1990) Book review: Development of forests and forestry Silva Fennica vol. 24 no. 4 article id 5435 (remove) | Edit comment
San José M. C., Romero L. et al. (2012) Effect of indole-3-butyric acid on root formatio.. Silva Fennica vol. 46 no. 5 article id 916 (remove) | Edit comment
Your search results