Concentration of the phytotoxic air pollutant, ozone (O3) is continually increasing in the lower layer of the troposphere. The purpose of this study was to compare performance of pine sawflies on Scots pine (Pinus sylvestris L.) seedlings in ambient and future levels of ozone. Scots pine seedlings were grown in field fumigation system where the ozone doses in fumigated plots were 1.5–1.6 times the ambient level. Larvae of the European pine sawfly (Neodiprion sertifer Geoffroy and Gilpinia pallida Klug) were reared on the foliage of Scots pine. The levels of resin acids and monoterpenes in foliage were analysed. There were no significant effects of ozone fumigation on sawfly performance or levels of defence compounds in pine foliage. The results suggest that the elevated ozone concentrations do not strongly affect the needle quality of young Scots pine and the importance of these two diprionid sawfly species forest pests.
Unequivocal evidence on the antagonistic effects of endophytic fungi associated with woody plants against insect herbivores has been documented in only a few cases so far. Experimental evidence of the significance of needle endophytes to coniferous trees has remained scant because it is difficult to obtain trees with needles free of endophytes that could be used as comparable controls for trees infected with endophytes. Previously we reported a new methodology to get Norway spruce (Picea abies (L.) Karst.) saplings without needle endophytes and to inoculate them with a needle endophyte Lophodermium piceae (Fuckel) Höhn. Here we describe the first trial where spruce saplings with and without needle endophytes were provided as substrate for insect larvae. We transferred larvae of two sawfly species, Neodiprion sertifer Geoffroy and Gilpina pallida Klug, to the seedlings. Even though the main host of these sawfly species is not Norway spruce, but Scots pine (Pinus sylvestris L.), they are also known to occasionally feed on spruce. In this experiment the larvae did not develop to pupae with the provided spruce substrate but consumed measurable amounts of needles. No significant difference was found between the extent of needles consumed by either of the two sawfly species in inoculated and uninoculated saplings.