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Articles containing the keyword 'colchicine'

Category : Article

article id 5423, category Article
Eliisa Särkilahti, Terho Valanne. (1990). Induced polyploidy in Betula. Silva Fennica vol. 24 no. 2 article id 5423. https://doi.org/10.14214/sf.a15577
Keywords: Betula; Finland; breeding; colchicine; polyploidy
Abstract | View details | Full text in PDF | Author Info

Seeds of ten different Betula species were treated with colchicine solution during germination, to induce duplication of the chromosome set. The species included in the study were B. pendula, B. pubescens, B. papyrifera subcordata, B. papyrifera papyrifera, B. papyrifera occidentalis, B. populifolia, B. alleghaniensis, B.n igra, B. glandulosa and B. nana. The total number of individually labelled, colchicine-treated trees was 1,550. Colchicine treatment induced changes in morphological features, especially in the leaves. These features proved to be good indicators of polyploidization. The experiments produced 687 polyploid trees, 287 of which are still alive. The polyploid Betula trees offer possibilities of studying the significance of the genome dosage for the growth, breeding, adaptability and evolution of Betula.

The PDF includes an abstract in Finnish.

  • Särkilahti, E-mail: es@mm.unknown (email)
  • Valanne, E-mail: tv@mm.unknown

Category : Research article

article id 10132, category Research article
Yan Liu, Yuan Zhang, Qing Zhou, Jian Wu, Pingdong Zhang. (2019). Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa. Silva Fennica vol. 53 no. 2 article id 10132. https://doi.org/10.14214/sf.10132
Keywords: colchicine-induced 2n pollen; pollen production; pollen viability; pollen germination rate; triploid
Highlights: The number of colchicine injections and the meiotic stage at which they were administered both had a significant effect on the occurrence rate of induced 2n pollen in Populus tomentosa; Treating male buds with 5000 ppm colchicine solution during meiosis led to a significant decrease in pollen production; Colchicine injection could induce P. tomentosa to produce 2n pollen and did not lead to dysfunction of induced diploid pollen.
Abstract | Full text in HTML | Full text in PDF | Author Info

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination in vitro and crossing induced pollen with normal gametes to produce triploid in Populus tomentosa Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (p < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce P. tomentosa to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.

  • Liu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 342767649@qq.com
  • Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 409192881@qq.com
  • Zhou, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 876034493@qq.com
  • Wu, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: 1269485709@qq.com
  • Zhang, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China; College of Bioscience and Biotechnology, Beijing Forestry University, Beijing 100083, China E-mail: zhangpd@bjfu.edu.cn (email)
article id 932, category Research article
Jun Wang, Le Shi, Shaoyu Song, Ju Tian, Xiangyang Kang. (2013). Tetraploid production through zygotic chromosome doubling in Populus. Silva Fennica vol. 47 no. 2 article id 932. https://doi.org/10.14214/sf.932
Keywords: Populus; colchicine; high temperature; seed hair; tetraploid induction; zygote
Abstract | Full text in HTML | Full text in PDF | Author Info
The most direct approach in breeding triploid Populus is crossing allotetraploids with diploids. However, the lack of allotetraploid Populus restricts application of this approach. In this investigation, zygotic chromosome doubling was induced with colchicine and high temperatures to produce ((Populus pseudo-simonii × P. nigra ‘Zheyin3#’) × (P. × beijingensis)) allotetraploids. We screened 6 and 25 tetraploid individual offspring from the colchicine and high-temperature treatments respectively, indicating that both colchicine and high temperature are effective for tetraploid production by zygotic chromosome doubling of Populus. Developmental characteristics of seed hairs in the ovaries were temporally associated with zygotic development, which was used to successfully guide the colchicine and high-temperature treatments. During certain stages of hair development, the efficiency of tetraploid production was significantly high. However, efficiency of production was not significantly influenced by other factors, i.e. colchicine concentration, temperature or duration of high-temperature treatment. Size and frequency of leaf stomata between tetraploid and diploid plants were significantly different, suggesting that this character can be altered via genomic increase in material. The allotetraploids produced in this investigation, having different genotypes, provide important parental germplasms for further triploid breeding.
  • Wang, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: wangjun@bjfu.edu.cn (email)
  • Shi, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: 7320932@qq.com
  • Song, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: angela-song@foxmail.com
  • Tian, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: 1215813245@qq.com
  • Kang, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China E-mail: kangxy@bjfu.edu.cn

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