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Articles containing the keyword 'satellite'

Category : Article

article id 5219, category Article
Simo Poso, Tuomas Häme, Raito Paananen. (1984). A method for estimating the stand characteristics of a forest compartment using satellite imagery. Silva Fennica vol. 18 no. 3 article id 5219. https://doi.org/10.14214/sf.a15398
Keywords: forest inventories; remote sensing; satellite imagery; survey by stands; compartmentwise forest inventories; relascope plots
Abstract | View details | Full text in PDF | Author Info

The paper presents a method based on two phase sampling and applicable to forest inventories. The first phase estimates are obtained from satellite imagery and, if required, from extra material such as maps. Second phase estimates are measured in the field. The method is flexible and also applicable to compartmentwise forest inventories. The experiments were based on six study areas with 439 relascope plots. The correlation coefficients between first and second stage estimates varied largely according to the study area.

The PDF includes a summary in Finnish.

  • Poso, E-mail: sp@mm.unknown (email)
  • Häme, E-mail: th@mm.unknown
  • Paananen, E-mail: rp@mm.unknown

Category : Article

article id 7524, category Article
Jari Varjo. (1997). Change detection and controlling forest information using multi-temporal Landsat TM imagery. Acta Forestalia Fennica no. 258 article id 7524. https://doi.org/10.14214/aff.7524
Keywords: forest inventory; change detection; continuous updating; satellite image; radiometric calibration; stand information; nonparametric discrimination
Abstract | View details | Full text in PDF | Author Info

A method was developed for relative radiometric calibration of single multitemporal Landsat TM image, several multitemporal images covering each other, and several multitemporal images covering different geographical locations. The radiometrically calibrated different images were used for detecting rapid changes on forest stands. The nonparametric Kernel method was applied for change detection. The accuracy of the change detection was estimated by inspecting the image analysis results in field.

The change classification was applied for controlling the quality of the continuously updated forest stand information. The aim was to ensure that all the manmade changes and any forest damages were correctly updated including the attribute and stand delineation information. The image analysis results were compared with the registered treatments and the stand information base. The stands with discrepancies between these two information sources were recommended to be field inspected.

  • Varjo, E-mail: jv@mm.unknown (email)
article id 7675, category Article
Erkki Tomppo. (1992). Satellite image aided forest site fertility estimation for forest income taxation. Acta Forestalia Fennica no. 229 article id 7675. https://doi.org/10.14214/aff.7675
Keywords: site quality; discriminant analysis; forest taxation; satellite images; segmentation; logistic regression analysis; Markov random field
Abstract | View details | Full text in PDF | Author Info

Two operative forest site class estimation methods utilizing satellite images have been developed for forest income taxation purposes. For this, two pixelwise classification methods and two post-processing methods for estimating forest site fertility are compared using different input data. The pixelwise methods are discriminant analysis, based on generalized squared distances, and logistic regression analysis. The results of pixelwise classifications are improved either with mode filtering within forest stands or assuming a Markov random field type dependence between pixels. The stand delineation is obtained by using ordinary segmentation techniques. Optionally, known stand boundaries given by the interpreter can be applied. The spectral values of images are corrected using a digital elevation model of the terrain. Some textural features are preliminary tested in classification. All methods are justified by using independent test data.

A test of the practical methods was carried out and a cost-benefit analysis computed. The estimated cost saving in site quality classification varies from 14% to 35% depending on the distribution of the site classes of the area. This means a saving of about 2.0–4.5 million FMK per year in site fertility classification for income taxation purposes. The cost savings would rise even to 60% if that version of the method were chosen where field checking is totally omitted. The classification accuracy at the forest holding level would still be similar to that of traditional method.

The PDF includes a summary in Finnish.

  • Tomppo, E-mail: et@mm.unknown (email)
article id 7668, category Article
Tuomas Häme. (1991). Spectral interpretation of changes in forest using satellite scanner images. Acta Forestalia Fennica no. 222 article id 7668. https://doi.org/10.14214/aff.7668
Keywords: remote sensing; forests; satellite image; changes; interpretation
Abstract | View details | Full text in PDF | Author Info

Spectral characteristics of rapid changes in forest and the spectral separability of change categories were studied through the analysis of satellite scanner images. A computational model of the spectral reflectance of a Scots pine (Pinus sylvestris L.) stand as a function of time was constructed and compared with empirical data. The study area, centred at 61°51’ N, 24°22’ E, was located in boreal forest in Southern Finland. Ground truth data consisted of forest stands and sample plots. Spectral data comprised multitemporal Landsat Thematic Mapper and Spot images as well as spectroradiometer measurements. The separability of the changes was tested with statistical tests and classifications. The separability varied according to the change category. A scheme for fully automated change monitoring system was presented.

The PDF includes a summary in Finnish.

  • Häme, E-mail: th@mm.unknown (email)

Category : Research article

article id 10460, category Research article
Hanna Lundmark, Lars Östlund, Torbjörn Josefsson. (2021). Continuity forest or second-generation forest? Historic aerial photos provide evidence of early clear-cutting in northern Sweden. Silva Fennica vol. 55 no. 1 article id 10460. https://doi.org/10.14214/sf.10460
Keywords: continuous cover forestry; forest history; aerial photography; satellite image; clear-cutting; clear-felling
Highlights: In the early 1900s clear-cutting had been applied to 10% of the forest land in the study area situated in Västernorrland province, northern Sweden; By the end of the 1940s 40% of the study area had been clear-cut and constituted second-generation forest; 50–70 years is too short of a time frame for assessing the continuity of a forest in the study area.
Abstract | Full text in HTML | Full text in PDF | Author Info

Modern forestry, which mainly consists of clear-cutting, is one of the most important factors influencing today’s boreal forests. In Sweden, the breaking point for modern forestry is generally considered to be around 1950. Recently, our common knowledge of the implementation of clear-cutting in Sweden has increased, and new research indicates that clear-cutting systems were already applied before the 1950s. In this case study, we used aerial photographs from the 1940s to analyze the extent of contemporaneous clear-cuts and even-aged young forests in an area in northern Sweden. Our results show that almost 40% of the study area had already been clear-cut by the end of the 1940s, but also that clear-cutting had been applied to 10% of the forest land in the early 1900s. This implies that the historical development of forestry in northern Sweden is more complex than previously thought, and that certain proportions of the forest land were already second-generation forests in the 1950s. Our results have implications for the use of concepts such as “continuity forest”, suggesting that this concept should employ a time frame of at least 100 years.

  • Lundmark, Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden ORCID https://orcid.org/0000-0001-8402-7152 E-mail: hanna.lundmark@slu.se
  • Östlund, Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden ORCID https://orcid.org/0000-0002-7902-3672 E-mail: lars.ostlund@slu.se
  • Josefsson, Department of Forest Ecology and Management, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden ORCID https://orcid.org/0000-0002-8734-5778 E-mail: torbjorn.josefsson@slu.se (email)
article id 10360, category Research article
Mikko Kukkonen, Eetu Kotivuori, Matti Maltamo, Lauri Korhonen, Petteri Packalen. (2021). Volumes by tree species can be predicted using photogrammetric UAS data, Sentinel-2 images and prior field measurements. Silva Fennica vol. 55 no. 1 article id 10360. https://doi.org/10.14214/sf.10360
Keywords: forest inventory; satellite image; open data; drone; stereo matching; unmanned aircraft system
Highlights: A UAS-based species-specific forest inventory approach that avoids new field measurements is presented; Models were constructed using previously measured training plots and remotely sensed data; Bi-seasonal Sentinel-2 data were beneficial in the prediction of species-specific volumes; RMSE values associated with the prediction of volumes by tree species and total volume at the validation plot level were 33.4–62.6% and 9.0%, respectively.
Abstract | Full text in HTML | Full text in PDF | Author Info

Photogrammetric point clouds obtained with unmanned aircraft systems (UAS) have emerged as an alternative source of remotely sensed data for small area forest management inventories (FMI). Nonetheless, it is often overlooked that small area FMI require considerable field data in addition to UAS data, to support the modelling of forest attributes. In this study, we propose a method whereby tree volumes by species are predicted with photogrammetric UAS data and Sentinel-2 images, using models fitted with airborne laser scanning data. The study area is in a managed boreal forest area in Eastern Finland. First, we predicted total volume with UAS point cloud metrics using a prior regression model fitted in another area with ALS data. Tree species proportions were then predicted by k nearest neighbor (k-NN) imputation based on bi-seasonal Sentinel-2 images without measuring new field plot data. Species-specific volumes were then obtained by multiplying the total volume by species proportions. The relative root mean square error (RMSE) values for total and species-specific volume predictions at the validation plot level (30 m × 30 m) were 9.0%, and 33.4–62.6%, respectively. Our approach appears promising for species-specific small area FMI in Finland and in comparable forest conditions in which suitable field plots are available.

  • Kukkonen, University of Eastern Finland, School of Forest Sciences, P.O. Box 111, FI-80101 Joensuu, Finland E-mail: mikko.kukkonen@uef.fi (email)
  • Kotivuori, University of Eastern Finland, School of Forest Sciences, P.O. Box 111, FI-80101 Joensuu, Finland E-mail: eetu.kotivuori@uef.fi
  • Maltamo, University of Eastern Finland, School of Forest Sciences, P.O. Box 111, FI-80101 Joensuu, Finland E-mail: matti.maltamo@uef.fi
  • Korhonen, University of Eastern Finland, School of Forest Sciences, P.O. Box 111, FI-80101 Joensuu, Finland E-mail: lauri.korhonen@uef.fi
  • Packalen, University of Eastern Finland, School of Forest Sciences, P.O. Box 111, FI-80101 Joensuu, Finland E-mail: petteri.packalen@uef.fi
article id 10347, category Research article
Matti Katila, Tuomas Rajala, Annika Kangas. (2020). Assessing local trends in indicators of ecosystem services with a time series of forest resource maps. Silva Fennica vol. 54 no. 4 article id 10347. https://doi.org/10.14214/sf.10347
Keywords: National Forest Inventory; satellite images; k-nearest neighbour estimation; Landsat; Mann-Kendall test; multitemporal inventory data; temporal trend analysis
Highlights: Untitled Document Contextual Mann-Kendall test detects significant trends in time-series of forest maps; Trends become more consistent as the areal unit size used for test input increases; Changes in different scales reflect different phenomena in forests; Significant trends were detected even after multiple testing correction.
Abstract | Full text in HTML | Full text in PDF | Author Info

Since the 1990’s, forest resource maps and small area estimates have been produced by combining national forest inventory (NFI) field plot data, optical satellite images and numerical map data using a non-parametric k-nearest neighbour method. In Finland, thematic maps of forest variables have been produced by the means of multi-source NFI (MS-NFI) for eight to ten times depending on the geographical area, but the resulting time series have not been systematically utilized. The objective of this study was to explore the possibilities of the time series for monitoring the key ecosystem condition indicators for forests. To this end, a contextual Mann-Kendall (CMK) test was applied to detect trends in time-series of two decades of thematic maps. The usefulness of the observed trends may depend both on the scale of the phenomena themselves and the uncertainties involved in the maps. Thus, several spatial scales were tested: the MS-NFI maps at 16 × 16 m2 pixel size and units of 240 × 240 m2, 1200 × 1200 m2 and 12  000 × 12  000 m2 aggregated from the MS-NFI map data. The CMK test detected areas of significant increasing trends of mean volume on both study sites and at various unit sizes except for the original thematic map pixel size. For other variables such as the mean volume of tree species groups, the proportion of broadleaved tree species and the stand age, significant trends were mostly found only for the largest unit size, 12  000 × 12  000 m2. The multiple testing corrections decreased the amount of significant p-values from the CMK test strongly. The study showed that significant trends can be detected enabling indicators of ecosystem services to be monitored from a time-series of satellite image-based thematic forest maps.

  • Katila, Natural Resources Institute Finland (Luke), Bioeconomy and environment, Latokartanonkaari 9, FI-00790 Helsinki, Finland; ORCID https://orcid.org/0000-0001-6946-5736 E-mail: matti.katila@luke.fi (email)
  • Rajala, Natural Resources Institute Finland (Luke), Natural resources, Latokartanonkaari 9, FI-00790 Helsinki, Finland E-mail: tuomas.rajala@luke.fi
  • Kangas, Natural Resources Institute Finland (Luke), Bioeconomy and environment, Yliopistokatu 6, FI-80100 Joensuu, Finland ORCID https://orcid.org/0000-0002-8637-5668 E-mail: annika.kangas@luke.fi
article id 1683, category Research article
Karol Przeździecki, Jarosław Zawadzki, Chris Cieszewski, Pete Bettinger. (2017). Estimation of soil moisture across broad landscapes of Georgia and South Carolina using the triangle method applied to MODIS satellite imagery. Silva Fennica vol. 51 no. 4 article id 1683. https://doi.org/10.14214/sf.1683
Keywords: soil moisture; remotely sensed imagery; satellite observations of forests; triangle method; TVDI; MODIS
Highlights: Temperature vegetation dryness indices were calculated from MODIS satellite imagery to estimate subsurface soil moisture at different depths using the triangle method; Observations were carried out over the vast areas of Georgia and South Carolina, USA, covered with diverse land uses that, included dense forests and agricultural areas; The triangle method may be useful in forestry management applications where the productivity potential of a region and the hydrologic role of forests in that region are of concern.
Abstract | Full text in HTML | Full text in PDF | Author Info

We describe here a study based on analysis of vegetation indices and land surface temperatures, which provides relevant information for estimating soil moisture at regional scales. Through an analysis of MODIS satellite imagery and in situ moisture data, the triangle method was used to develop a conceptual land surface temperature−vegetation index model, and spatial temperature-vegetation dryness index (TVDI) values to describe soil moisture relationships for a broad landscape. This study was situated mainly within two states of the southern United States (Georgia and South Carolina). The total study area was about 30 million hectares. The analyses were conducted using information gathered from the 2009 growing season (from the end of March to September). The results of the study showed that soil moisture content was inversely proportional to TVDI, and that TVDI based on the normalized difference vegetation index (NDVI) had a slightly higher correlation with soil moisture than TVDI based on the enhanced vegetation index (EVI).

  • Przeździecki, Warsaw University of Technology, Faculty of Building Services, Hydro and Environmental Engineering, 00-653, Nowowiejska 20, Warszawa, Poland ORCID http://orcid.org/0000-0002-2275-5223 E-mail: karol_przezdziecki@is.pw.edu.pl (email)
  • Zawadzki, Warsaw University of Technology, Faculty of Building Services, Hydro and Environmental Engineering, 00-653, Nowowiejska 20, Warszawa, Poland ORCID http://orcid.org/0000-0003-2842-0018 E-mail: j.j.zawadzki@gmail.com
  • Cieszewski, University of Georgia, Warnell School of Forestry and Natural Resources, 180 E Green St, Athens, GA 30602, USA ORCID http://orcid.org/0000-0003-2842-4406 E-mail: thebiomat@gmail.com
  • Bettinger, University of Georgia, Warnell School of Forestry and Natural Resources, 180 E Green St, Athens, GA 30602, USA ORCID http://orcid.org/0000-0002-5454-3970 E-mail: pbettinger@warnell.uga.edu
article id 7001, category Research article
Fei-Fei Zhai, Jun-Xiang Liu, Zhen-Jian Li, Jin-Mei Mao, Yong-Qiang Qian, Lei Han, Zhen-Yuan Sun. (2017). Assessing genetic diversity and population structure of Salix viminalis across Ergun and West Liao basin. Silva Fennica vol. 51 no. 3 article id 7001. https://doi.org/10.14214/sf.7001
Keywords: genetic variation; microsatellites; geographic barrier; basket willow; common osier
Highlights: High genetic diversity and moderate population differentiation were detected in twelve Salix viminalis populations; Populations from Ergun basin presented higher genetic diversity than populations from West Liao basin; Populations with short distances presented low differentiation.
Abstract | Full text in HTML | Full text in PDF | Author Info

Salix viminalis L. is an important shrub that has potential for use as a bioenergy crop, for phytoremediation of heavy metal contaminated soil and sewage sludge treatment. It is mainly distributed in the northeast of China, but the species has not yet been used a resource here. We examined the genetic diversity and population structure of populations from the Ergun basin and West Liao basin using 20 microsatellite markers. A high level of genetic diversity (Na = 16.45, He = 0.742) was detected for S. viminalis, and populations from the Ergun basin exhibited higher genetic diversity and private alleles numbers than the West Liao basin. The 12 populations could be divided into two clusters by both Bayesian analysis and UPGMA clustering which were consistent with the populations derived from the two basins. Moderate population differentiation (FST = 0.076) was shown in S. viminalis, and AMOVA analysis confirmed that most of the genetic variation (86.13%) was attributed to individual differences within populations, while 11.49% was attributed to differences between basins and 2.38% to differences within each basin. Significant correlations of FST/(1–FST) with log (geographic distance) among 12 populations (r = 0.634, p < 0.00) and 10 populations within the Ergun basin (r = 0.482, p = 0.0002) indicated that geographical distance was the principal factor influencing genetic structure. As most of genetic variation exist within populations, so protection measures should be focused on populations with higher genetic diversity and unique alleles, such as Tuli, Mordaga downstream, Zhadun1 and Genhe.

  • Zhai, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China; School of Architectural and Artistic Design, Henan Polytechnic University, Century Avenue, Jiaozuo, Henan, 454000, PR China E-mail: lkyzff@163.com
  • Liu, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China E-mail: liu-jx295@163.com
  • Li, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China E-mail: zhenjianli@163.com
  • Mao, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China; Research Institute of Economic Forest, Xinjiang Academy of Forestry, Anjunanlu, Urumqi, Xinjiang, PR China E-mail: 350512173@qq.com
  • Qian, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China E-mail: qianyq@caf.ac.cn
  • Han, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China E-mail: hdd@caf.ac.cn
  • Sun, State Key Laboratory of Tree Genetics and Breeding; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Haidian District, Beijing, 10091, PR China E-mail: lkyszy@126.com (email)
article id 1721, category Research article
Anna Hebda, Błażej Wójkiewicz, Witold Wachowiak. (2017). Genetic characteristics of Scots pine in Poland and reference populations based on nuclear and chloroplast microsatellite markers. Silva Fennica vol. 51 no. 2 article id 1721. https://doi.org/10.14214/sf.1721
Keywords: Pinus sylvestris; phylogeography; conifers; genetic divergence; population structure; microsatellite markers; neutral variation
Highlights: Similar genetic variation was found between Polish Scots pine populations from a wide variety of habitats based on nSSR and cpSSR markers; Homogeneity was observed in the genetic structures of Polish and Finnish populations from the continuous pine range; Genetic differentiation in microsatellite markers was identified only when populations from the central pine distribution were compared to the marginal stands.
Abstract | Full text in HTML | Full text in PDF | Author Info

Polymorphisms at a set of eighteen nuclear (nSSR) and chloroplast (cpSSR) microsatellite loci were investigated in sixteen populations of Scots pine (Pinus sylvestris L.) derived from the provenance trial experiment and representative of the species distribution range and climatic zones in Poland. The patterns of genetic variation were compared to the reference samples from the species distribution in Europe and Asia. A similar level of genetic variation and no evidence of population structure was found among the Polish stands. They showed genetic similarity and homogenous patterns of allelic frequency spectra compared to the Northern European populations. Those populations were genetically divergent compared to the marginal populations from Turkey, Spain and Scotland. The population structure patterns reflect the phylogeography of the species and the divergence of populations that most likely do not share recent history. As the analysed provenance trial populations from Poland are diverged in phenotypic traits but are genetically similar, they could be used to test for selection at genomic regions that influence variation in quantitative traits.

  • Hebda, University of Agriculture in Krakow, Faculty of Forestry, Institute of Forest Ecology and Silviculture, Department of Genetics and Forest Tree Breeding, 29 Listopada 46, 31-425 Kraków, Poland ORCID http://orcid.org/0000-0002-3149-8644 E-mail: ana.hebda@gmail.com (email)
  • Wójkiewicz, Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland E-mail: bwojkiew@man.poznan.pl
  • Wachowiak, Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland; Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland E-mail: witoldw@man.poznan.pl
article id 1705, category Research article
Sulmaz Janfaza, Hamed Yousefzadeh, Seyed Mohammad Hosseini Nasr, Roberto Botta, Asad Asadi Abkenar, Daniela Torello Marinoni. (2017). Genetic diversity of Castanea sativa an endangered species in the Hyrcanian forest. Silva Fennica vol. 51 no. 1 article id 1705. https://doi.org/10.14214/sf.1705
Keywords: population structure; microsatellite markers; sweet chestnut
Highlights: This is the first report of genetic diversity of the few remaining populations of C. sativa in the southern limits of its distribution in Eurasia; Low genetic diversity and high genetic differentiation among small isolated populations of C. sativa with low geographical distance represent serious genetic erosion indicators in the Hyrcanian forest, even hinting at an ongoing extinction vortex.
Abstract | Full text in HTML | Full text in PDF | Author Info

Castanea sativa Mill. is one of the most endangered tree species in Iran where it is represented by small fragmented populations in the north of the country. 18 simple sequence repeat (SSR) loci (10 nuclear and 8 chloroplastic) were used to evaluate the genetic diversity and population structure of C. sativa from the Hyrcanian forest. For nuclear SSR, the number of alleles detected per locus ranged from 1 to 5 and observed heterozygosity (HO) was between 0.125 and 1.000. Analysis of molecular variance (AMOVA) indicated a high level of variation within populations (84%) and low levels between populations (16%). Based on structure analysis, the four studied populations were divided into two main clusters that have genetic distance Fst = 0.3. The Shafaroud population was separated in the first cluster, Siyahmazgi, Qalehroudkhan and Veysroud were placed in the second cluster. The UPGMA analysis confirmed the results of Structure analysis, separating the Shafaroud population from the others. The 8 chloroplast SSR loci used to screen the populations showed no polymorphism. In General, low nuclear genetic diversity, no polymorphism in cpDNA and considerable genetic differentiation among populations in short geographical distance represent a serious genetic erosion threat for C. sativa in the Hyrcanian forest, even hinting at an ongoing extinction vortex. Therefore, due to significant decline in genetic diversity, it is essential to introduce constraints protection upon the areas of distribution of all four populations of this species in Iran.

  • Janfaza, Department of Forestry, Sari University of Agriculture Sciences and Natural Resources, Km 9 Darya Road, P.O. Box 578, Mazandaran, Iran E-mail: aristocratka_b@yahoo.com
  • Yousefzadeh, Department of Environment, Faculty of Natural Resources and Marine Science ,Tarbiat Modares University, Noor, Mazandaran, Iran E-mail: h.yousefzadeh@modares.ac.ir (email)
  • Hosseini Nasr, Department of Forestry, Sari University of Agriculture Sciences and Natural Resources, Km 9 Darya Road, P.O. Box 578, Mazandaran, Iran E-mail: s.hosseini@sanru.ac.ir
  • Botta, Dipartimento di Scienze Agrarie Forestali e Alimentari, DISAFA, Università di Torino, Via Verdi 8, 10124 Torino, Italy E-mail: roberto.botta@unito.it
  • Asadi Abkenar, Agricultural Research, Education and Extension Organization (AREEO) of IRAN, Agricultural Biotechnology Research Institute of Iran (RBRII), Branch of North Region, Guilan, Rasht, Iran E-mail: asadiabkenarasad@gmail.com
  • Marinoni, Dipartimento di Scienze Agrarie Forestali e Alimentari, DISAFA, Università di Torino, Via Verdi 8, 10124 Torino, Italy E-mail: daniela.marinoni@unito.it
article id 1510, category Research article
Tähti Pohjanmies, Sakina Elshibli, Pertti Pulkkinen, Mari Rusanen, Pekka Vakkari, Helena Korpelainen, Tomas Roslin. (2016). Fragmentation-related patterns of genetic differentiation in pedunculate oak (Quercus robur) at two hierarchical scales. Silva Fennica vol. 50 no. 2 article id 1510. https://doi.org/10.14214/sf.1510
Keywords: marginal populations; Finland; genetic diversity; microsatellites
Highlights: While long-lived, widespread tree species should be resistant to genetic impoverishment, we detected high differentiation among populations and pronounced genetic structure within populations of Quercus robur in Finland; These patterns seem indicative of population processes active at range margins, and of disequilibrium following historic habitat change; Preservation of remaining genetic variation is thus important in the conservation of Q. robur.
Abstract | Full text in HTML | Full text in PDF | Author Info

Populations at species’ range margins are expected to show lower genetic diversity than populations at the core of the range. Yet, long-lived, widespread tree species are expected to be resistant to genetic impoverishment, thus showing comparatively high genetic diversity within populations and low differentiation among populations. Here, we study the distribution of genetic variation in the pedunculate oak (Quercus robur L.) at its range margin in Finland at two hierarchical scales using 15 microsatellite loci. At a regional scale, we compared variation within versus among three oak populations. At a landscape scale, we examined genetic structuring within one of these populations, growing on an island of ca 5 km2. As expected, we found the majority of genetic variation in Q. robur to occur within populations. Nonetheless, differentiation among populations was markedly high (FST = 0.12) compared with values reported for populations of Q. robur closer to the core of its range. At the landscape level, some spatial and temporal sub-structuring was observed, likely explained by the history of land-use on the island. Overall, Q. robur fulfils the expectation of the central-marginal hypothesis of high differentiation among marginal populations, but the notable population differentiation has most likely been influenced also by the long, ongoing fragmentation of populations. Finnish oak populations may still be adjusting to the drastic habitat changes of the past centuries. Preservation of genetic variation within the remaining stands is thus an important factor in the conservation of Q. robur at its range margin.

  • Pohjanmies, University of Helsinki, Department of Agricultural Sciences, Spatial Foodweb Ecology Group, P.O. Box 27, FI-00014 University of Helsinki, Finland; University of Jyväskylä, Department of Biological and Environmental Sciences, P.O. Box 35, FI-40014 University of Jyväskylä, Finland E-mail: tahti.t.pohjanmies@jyu.fi (email)
  • Elshibli, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland; University of Helsinki, Department of Agricultural Sciences, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: sakina.elshibli@helsinki.fi
  • Pulkkinen, Natural Resources Institute Finland (Luke), Green technology, Haapastensyrjäntie 34, FI-12600 Läyliäinen, Finland E-mail: pertti.pulkkinen@luke.fi
  • Rusanen, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: mari.rusanen@luke.fi
  • Vakkari, Natural Resources Institute Finland (Luke), Green technology, P.O. Box 18, FI-01301 Vantaa, Finland E-mail: pekka.vakkari@luke.fi
  • Korpelainen, University of Helsinki, Department of Agricultural Sciences, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: helena.korpelainen@helsinki.fi
  • Roslin, University of Helsinki, Department of Agricultural Sciences, Spatial Foodweb Ecology Group, P.O. Box 27, FI-00014 University of Helsinki, Finland; Swedish University of Agricultural Sciences, Department of Ecology, P.O. Box 7044, SE-750 07 Uppsala, Sweden E-mail: tomas.roslin@helsinki.fi
article id 905, category Research article
Katarzyna A. Jadwiszczak, Danuta Drzymulska, Agata Banaszek, Piotr Jadwiszczak. (2012). Population history, genetic variation and conservation status of the endangered birch species Betula nana L. in Poland. Silva Fennica vol. 46 no. 4 article id 905. https://doi.org/10.14214/sf.905
Keywords: Betula nana; genetic diversity; microsatellites; conservation genetics; cpDNA; isolation
Abstract | View details | Full text in PDF | Author Info
The effective conservation of species requires data on the levels and distribution of genetic diversity within and among populations. In this study, we estimated the genetic variation in three isolated populations of Betula nana in Poland. An analysis of 11 nuclear microsatellites revealed moderate mean heterozygosities (HO=0.556, HE=0.562), low mean number of alleles per locus (A=4.57) and no inbreeding in the total sample. An M-ratio test indicated that each population had experienced a severe bottleneck in the past. Tests for heterozygosity excess revealed that a significant decrease in the numbers of individuals in two populations had occurred quite recently. The large number of private alleles and very restricted number of migrants between populations (Nm=0.35) strongly suggest that genetic drift and geographic isolation are the primary factors responsible for the reduction of genetic variation in the Polish populations of B. nana. We detected two cpDNA haplotypes in the study populations, which can be explained in terms of either the genetic drift acting on the relict localities or a postglacial recolonisation from distinct refugia. Palynological data indicated that one refugium could be located in the Carpathians and their northern foreland. The primary threat to B. nana in Poland is the overgrowth of its habitats by competing species, which has likely resulted in a lack of generative reproduction in the mountain populations.
  • Jadwiszczak, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: kszalaj@uwb.edu.pl (email)
  • Drzymulska, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: dd@nn.pl
  • Banaszek, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: ab@nn.pl
  • Jadwiszczak, Institute of Biology, University of Białystok, wierkowa 20B, 15-950 Białystok, Poland E-mail: pj@nn.pl
article id 456, category Research article
Sam B. Coggins, Nicholas C. Coops, Michael A. Wulder. (2010). Improvement of low level bark beetle damage estimates with adaptive cluster sampling. Silva Fennica vol. 44 no. 2 article id 456. https://doi.org/10.14214/sf.456
Keywords: forest inventory; adaptive cluster sampling; mountain pine beetle; object-based classification; high spatial resolution; satellite; digital aerial imagery
Abstract | View details | Full text in PDF | Author Info
Detection of low level infestation in forest stands is of principle importance to determine effective control strategies before the attack spread to large areas. Of particular concern is the ongoing mountain pine beetle, Dendroctonus ponderosae (Hopkins) epidemic, which has caused approximately 14 million hectares of damage to lodgepole pine (Pinus contorta Dougl. ex. Loud var. latifolia Engl.) forests in western Canada. At the stand level attacked trees are often difficult to locate and can remain undetected until the infestation has become established beyond a small number of trees. As such, methods are required to detect and characterise low levels of attack prior to infestation expansion, to inform management, and to aid mitigation activities. In this paper, an adaptive cluster sampling approach was applied to very fine-scale (20 cm) digital aerial imagery to locate mountain pine beetle damaged trees at the leading edge of the current infestation. Results indicated a mean number of 7.36 infested trees per hectare with a variance of 18.34. In contrast a non-adaptive approach estimated the mean number of infested trees in the same area to be 61.56 infested trees per hectare with a variance of 41.43. Using a relative efficiency estimator the adaptive cluster sampling approach was found to be over two times more efficient when compared to the non-adaptive approach.
  • Coggins, Department of Forest Resources Management, University of British Columbia, 2424 Main Mall, Vancouver, B.C., Canada V6T 1Z4 E-mail: scoggins@interchange.ubc.ca (email)
  • Coops, Department of Forest Resources Management, University of British Columbia, 2424 Main Mall, Vancouver, B.C., Canada V6T 1Z4 E-mail: ncc@nn.ca
  • Wulder, Canadian Forest Service (Pacific Forestry Centre), Natural Resources Canada, 506 West Burnside Rd., Victoria, B.C., Canada V8Z 1M5 E-mail: maw@nn.ca
article id 314, category Research article
Yuhua Wang, Helena Korpelainen, Chunyang Li. (2006). Microsatellite polymorphism in the edaphic spruce, Picea asperata, originating from the mountains of China. Silva Fennica vol. 40 no. 4 article id 314. https://doi.org/10.14214/sf.314
Keywords: genetic diversity; microsatellite polymorphism; micro-geographic differentiation; natural selection; mutation
Abstract | View details | Full text in PDF | Author Info
Microsatellite variation of Picea asperata Mast. originating from the mountains of China was investigated by analyzing variation at seven SSR loci in 250 individuals representing ten populations. A fair degree of genetic diversity and considerable population subdivision occurred with the mean gene diversity (H) of 0.707, and genetic distances among populations varying between 0.121 and 0.224 (FST) and between 0.100 and 0.537 (RST). However, inter-population genetic distances showed no correlation with geographic distances between the population sites. This ruled out a simple isolation by distance model and suggested that migration does not have a great impact. In fact, the amount of gene flow, detected using private alleles, was very low, equaling only 0.753. Allele permutation tests revealed that stepwise-like mutations, coupled with genetic drift, could contribute to population differentiation. Moreover, significant genetic differences between populations were detected at most loci. The results indicate that natural selection, presumably through environmental stress, may be one of the main factors causing micro-geographical differentiation in the genetic structure of P. asperata. Based on SSR genotypes, 70% of the 250 individuals were correctly classified into their sites of origin. This suggests that microsatellites (SSRs) are effective in distinguishing genotypes of P. asperata originating from diverse eco-geographical sites in China.
  • Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China E-mail: yw@nn.cn
  • Korpelainen, Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: hk@nn.fi
  • Li, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China E-mail: licy@cib.ac.cn (email)
article id 326, category Research article
Xuejiang Zhang, Helena Korpelainen, Chunyang Li. (2006). Microsatellite variation of Quercus aquifolioides populations at varying altitudes in the Wolong Natural Reserve of China. Silva Fennica vol. 40 no. 3 article id 326. https://doi.org/10.14214/sf.326
Keywords: genetic differentiation; genetic variation; altitudinal gradients; microsatellites; Quercus aquifolioides
Abstract | View details | Full text in PDF | Author Info
Genetic variation and differentiation were investigated among five natural populations of Quercus aquifolioides occurring along an altitudinal gradient that varied from 2000 to 3600 m above sea level in the Wolong Natural Reserve of China, by analyzing variation at six microsatellite loci. The results showed that the populations were characterized by relatively high intra-population variation with the average number of alleles equaling 11.33 per locus and the average expected heterozygosity (HE) being 0.779. The amount of genetic variation varied only little among populations, which suggests that the influence of altitude factors on microsatellite variation is limited. However, there is a significantly positive correlation between altitude and the number of low-frequency alleles (R2 = 0.97, P < 0.01), which indicates that Q. aquifolioides from high altitudes has more unique variation, possibly enabling adaptation to severe conditions. F statistics showed the presence of a slight deficiency of heterozygosity (FIS = 0.136) and a low level of differentiation among populations (FST = 0.066). The result of the cluster analysis demonstrated that the grouping of populations does not correspond to the altitude of the populations. Based on the available data, it is likely that the selective forces related to altitude are not strong enough to significantly differentiate the populations of Q. aquifolioides in terms of microsatellite variation.
  • Zhang, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, P. R. China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China E-mail: xz@nn.cn (email)
  • Korpelainen, Department of Applied Biology, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: hk@nn.fi
  • Li, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, P. R. China E-mail: licy@cib.ac.cn
article id 478, category Research article
Ronald E. McRoberts, Daniel G. Wendt, Greg C. Liknes. (2005). Stratified estimation of forest inventory variables using spatially summarized stratifications. Silva Fennica vol. 39 no. 4 article id 478. https://doi.org/10.14214/sf.478
Keywords: bias; precision; classified satellite imagery; Internet; variance
Abstract | View details | Full text in PDF | Author Info
Large area natural resource inventory programs typically report estimates for selected geographic areas such as states or provinces, counties, and municipalities. To increase the precision of estimates, inventory programs may use stratified estimation, with classified satellite imagery having been found to be an efficient and effective basis for stratification. For the benefit of users who desire additional analyses, the inventory programs often make data and estimation procedures available via the Internet. For their own analyses, users frequently request access to stratifications used by the inventory programs. When data analysis is via the Internet and stratifications are based on classifications of even medium resolution satellite imagery, the memory requirements for storing the stratifications and the online time for processing them may be excessive. One solution is to summarize the stratifications at coarser spatial scales, thus reducing both storage requirements and processing time. If the bias and loss of precision resulting from using summaries of stratifications is acceptably small, then this approach is viable. Methods were investigated for using summaries of stratifications that do not require storing and processing the entire pixel-level stratifications. Methods that summarized satellite image-based 30 m x 30 m pixel stratifications at spatial scales up to 2400 ha produced stratified estimates of the mean that were generally within 5-percent of estimates for the same areas obtained using the pixel stratifications. In addition, stratified estimates of variances using summarized stratifications realized nearly all the gain in precision that was obtained with the underlying pixel stratifications.
  • McRoberts, North Central Research Station, USDA Forest Service, 1992 Folwell Avenue, Saint Paul, Minnesota, USA 5510 E-mail: rmcroberts@fs.fed.us (email)
  • Wendt, Region 9, USDA Forest Service, 626 East Wisconsin Avenue, Milwaukee, Wisconsin 53202, USA E-mail: dgw@nn.us
  • Liknes, North Central Research Station, USDA Forest Service, 1992 Folwell Avenue, Saint Paul, Minnesota, USA 5510 E-mail: gcl@nn.us
article id 367, category Research article
Sakari Tuominen, Markus Haakana. (2005). Landsat TM imagery and high altitude aerial photographs in estimation of forest characteristics. Silva Fennica vol. 39 no. 4 article id 367. https://doi.org/10.14214/sf.367
Keywords: multi-source forest inventory; satellite imagery; high-altitude aerial photographs; image texture
Abstract | View details | Full text in PDF | Author Info
Satellite sensor data have traditionally been used in multi-source forest inventory for estimating forest characteristics. Their advantages generally are large geographic coverage and large spectral range. Another remote sensing data source for forest inventories offering a large geographic coverage is high altitude aerial photography. In high altitude aerial photographs the spectral range is very narrow but the spatial resolution is high. This allows the extraction of texture features for forest inventory purposes. In this study we utilized a Landsat 7 ETM satellite image, a photo mosaic composed of high altitude panchromatic aerial photographs, and a combination of the aforementioned in estimating forest attributes for an area covering approximately 281 000 ha in Forestry Centre Häme-Uusimaa in Southern Finland. Sample plots of 9th National Forest Inventory (NFI9) were used as field data. In the estimation, 6 Landsat 7 ETM image channels were used. For aerial photographs, 4 image channels were composed from the spectral averages and texture features. In combining both data sources, 6 Landsat channels and 3 aerial image texture channels were selected for the analysis. The accuracy of forest estimates based on the Landsat image was better than that of estimates based on high altitude aerial photographs. On the other hand, using the combination of Landsat ETM spectral features and textural features on high altitude aerial photographs improved the estimation accuracy of most forest attributes.
  • Tuominen, Finnish Forest Research Institute, Unioninkatu 40 A, FI-00170 Helsinki, Finland E-mail: sakari.tuominen@metla.fi (email)
  • Haakana, Finnish Forest Research Institute, Unioninkatu 40 A, FI-00170 Helsinki, Finland E-mail: mh@nn.fi
article id 587, category Research article
Erkki Tomppo, Kari T. Korhonen, Juha Heikkinen, Hannu Yli-Kojola. (2001). Multi-source inventory of the forests of the Hebei Forestry Bureau, Heilongjiang, China. Silva Fennica vol. 35 no. 3 article id 587. https://doi.org/10.14214/sf.587
Keywords: models; forest inventory; satellite images; k-nearest neighbour method; China
Abstract | View details | Full text in PDF | Author Info
A multi-source forest inventory method is applied to the estimation of forest resources in the area of the Hebei Forest Bureau in Heilongjiang province in North-East China. A stratified systematic cluster sampling design was utilised in field measurements. The design was constructed on the basis of information from earlier stand-level inventories, aerial orthophotographs, experiences from other sampling inventories and the available budget. Sample tree volumes were estimated by means of existing models. New models were constructed and their parameters estimated for tallied tree volumes and volume increments. The estimates for the area of the Bureau were computed from field measurements, and for the areas of the forest farms estimated from field measurements and satellite images. A k-nearest neighbour method was utilised. This method employing satellite image data makes it possible to estimate all variables, particularly for smaller areas than that possible using field measurements only. The methods presented, or their modifications, could also be applied to the planning and realisation of forest inventories elsewhere in Temperate or Boreal zones. The inventory in question gave an estimate of 114 m3/ha (the multi-source inventory 119 m3/ha) instead of 72 m3/ha as previously estimated from available information. Totally nineteen tree species, genera of species or tree species groups were identified (Appendix 1). The forests were relatively young, 60% of them younger than 40 years and 85% younger than 60 years.
  • Tomppo, Finnish Forest Research Institute, Unioninkatu 40 A, FIN-00170 Helsinki, Finland E-mail: erkki.tomppo@metla.fi (email)
  • Korhonen, Finnish Forest Research Institute, Unioninkatu 40 A, FIN-00170 Helsinki, Finland E-mail: ktk@nn.fi
  • Heikkinen, Finnish Forest Research Institute, Unioninkatu 40 A, FIN-00170 Helsinki, Finland E-mail: jh@nn.fi
  • Yli-Kojola, Finnish Forest Research Institute, Unioninkatu 40 A, FIN-00170 Helsinki, Finland E-mail: hyk@nn.fi

Category : Review article

article id 923, category Review article
Katarzyna A. Jadwiszczak. (2012). What can molecular markers tell us about the glacial and postglacial histories of European birches? Silva Fennica vol. 46 no. 5 article id 923. https://doi.org/10.14214/sf.923
Keywords: Betula; cpDNA; glacial refugia; microsatellite; suture zone
Abstract | View details | Full text in PDF | Author Info
The last glaciation was one of the most severe of the Pleistocene epoch. The development of the Scandinavian ice sheet forced many species to reduce their ranges to areas with favourable climatic conditions. Most European species survived the Last Glacial Maximum in refugia in southern parts of Europe. Cold-tolerant species, such as birch trees and shrubs, could also inhabit western, eastern or central Europe. After climate warming, Holocene recolonisation began. This paper presents a comparative analysis of the genetic variation of four European Betula species to reconstruct their glacial and postglacial histories. Two chloroplast DNA haplotypes dominate within the ranges of all birch species, one haplotype is the most common in western and northwestern Europe, the second haplotype occurs mainly in the eastern and southeastern parts of the continent. This finding suggests that birches have recolonised Europe from the western and the eastern refugia, respectively. Most of Europe was likely populated from higher latitude refugia because there was no evidence of isolation by distance and weak genetic structures were detected. Similar patterns of haplotype distributions within Betula ranges indicate that postglacial recolonisation may be disturbed by interspecies hybridisation.
  • Jadwiszczak, Institute of Biology, University of Bialystok, wierkowa 20B, 15-950 Bialystok, Poland E-mail: kszalaj@uwb.edu.pl (email)

Category : Research note

article id 9986, category Research note
Ninni Saarinen, Joanne C. White, Michael A. Wulder, Annika Kangas, Sakari Tuominen, Ville Kankare, Markus Holopainen, Juha Hyyppä, Mikko Vastaranta. (2018). Landsat archive holdings for Finland: opportunities for forest monitoring. Silva Fennica vol. 52 no. 3 article id 9986. https://doi.org/10.14214/sf.9986
Keywords: National Forest Inventory; satellite; Landsat time series
Highlights: The 45-year Landsat archive contained 30 076 images for Finland by December 31, 2017; 16.3% of these were acquired within ±30 days of August 1 (northern hemisphere summer), have <70% cloud cover, and a 30 m spatial resolution; Using time series analyses, these data provide unique information that complements other datasets available for forest monitoring and assessment in Finland.
Abstract | Full text in HTML | Full text in PDF | Author Info

There is growing interest in the use of Landsat data to enable forest monitoring over large areas. Free and open data access combined with high performance computing have enabled new approaches to Landsat data analysis that use the best observation for any given pixel to generate an annual, cloud-free, gap-free, surface reflectance image composite. Finland has a long history of incorporating Landsat data into its National Forest Inventory to produce forest information in the form of thematic maps and small area statistics on a variety of forest attributes. Herein we explore the spatial and temporal characteristics of the Landsat archive in the context of forest monitoring in Finland. The United States Geological Survey Landsat archive holds a total of 30 076 images (1972–2017) for 66 scenes (each 185 km by 185 km in size) representing the terrestrial area of Finland, of which 93.6% were acquired since 1984 with a spatial resolution of 30 m. Approximately 16.3% of the archived images have desired compositing characteristics (acquired within August 1 ±30 days, <70% cloud cover, 30 m spatial resolution). Data from the Landsat archive can augment forest monitoring efforts in Finland, provide new information for science and applications, and enable retrospective, systematic analyses to characterize the development of Finnish forests over the past three decades. The capacity to monitor trends based upon this multi-decadal record with the addition of new measurements is of benefit to multisource inventories and offers nationally comprehensive spatially-explicit datasets for a wide range of stakeholders and applications.

  • Saarinen, Department of Forest Sciences, University of Helsinki, P.O. Box 27, FI-00014 University of Helsinki, Finland; School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland ORCID https://orcid.org/0000-0003-2730-8892 E-mail: ninni.saarinen@helsinki.fi (email)
  • White, Department of Forest Sciences, University of Helsinki, P.O. Box 27, FI-00014 University of Helsinki, Finland; Canadian Forest Service, (Pacific Forestry Center), Natural Resources Canada, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada ORCID http://orcid.org/0000-0003-4674-0373 E-mail: joanne.white@canada.ca
  • Wulder, Canadian Forest Service, (Pacific Forestry Center), Natural Resources Canada, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada ORCID https://orcid.org/0000-0002-6942-1896 E-mail: mike.wulder@canada.ca
  • Kangas, Natural Resources Institute Finland (Luke), Bioeconomy and environment, Yliopistokatu 6, FI-80100 Joensuu, Finland E-mail: annika.kangas@luke.fi
  • Tuominen, Natural Resources Institute Finland (Luke), Bioeconomy and environment, Latokartanonkaari 9, FI-00790 Helsinki, Finland E-mail: sakari.tuominen@luke.fi
  • Kankare, Department of Forest Sciences, University of Helsinki, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: ville.kankare@helsinki.fi
  • Holopainen, Department of Forest Sciences, University of Helsinki, P.O. Box 27, FI-00014 University of Helsinki, Finland E-mail: markus.holopainen@helsinki.fi
  • Hyyppä, Department of Remote Sensing and Photogrammetry, Finnish Geospatial Research Institute, National Land Survey of Finland, Geodeetinrinne 2, FI-02431 Masala, Finland E-mail: juha.hyyppa@nls.fi
  • Vastaranta, School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland ORCID https://orcid.org/0000-0001-6552-9122 E-mail: mikko.vastaranta@uef.fi
article id 5644, category Research note
Jiří Korecký, Jan Bílý, Petr Sedlák, Milan Lstibůrek. (2017). Innovative multiplex and its evaluation for effective genotyping of wild cherry. Silva Fennica vol. 51 no. 3 article id 5644. https://doi.org/10.14214/sf.5644
Keywords: genetic structure; microsatellites; Prunus avium; SSRs; pseudo 15-plex; one-run genotyping
Highlights: We present simple and cost-efficient method of SSR genotyping in cherry species; Seven of fifteen previously published primer sequences have been altered in order to produce complementary amplicons in pseudo 15-plex; The effectiveness and discriminatory power of established multiplex were verified by SSRs analysis of 48 wild cherry trees.
Abstract | Full text in HTML | Full text in PDF | Author Info

Trees from the family Rosaceae play an important role in forest and agricultural ecosystems. Therefore, they are often an object of interest for both forest and horticultural tree breeders. Here, we present the utilization of an effective microsatellite (SSRs) genotyping method for wild cherry (Prunus avium L.) and verified the discriminatory power of the presented multiplex by genotyping 48 genetically distinctive individuals (plus-trees). Concerned loci were previously proven to be cross-compatible among various cultivars of cherry, hence, the method could have a broader utilization beyond to the field of forestry.
Our technique is based on post-PCR processing of 15 polymorphic SSRs loci amplified in three multiplex reactions with fluorescently labeled primers (6-FAM, VIC, PET and NED). All PCR products could be pooled and analyzed simultaneously (pseudo 15-plex). In order to make this approach feasible, we redefined sequences of several primers. Thus, utilizing modified primers provides non-overlapping amplicons of each fluorescent dye.

  • Korecký, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech Republic ORCID http://orcid.org/0000-0001-7859-1750 E-mail: korecky@fld.czu.cz (email)
  • Bílý, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech Republic ORCID http://orcid.org/0000-0001-5794-0907 E-mail: bily@fld.czu.cz
  • Sedlák, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague, Czech Republic ORCID http://orcid.org/0000-0001-8016-8900 E-mail: sedlak@af.czu.cz
  • Lstibůrek, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech Republic ORCID http://orcid.org/0000-0002-6304-6669 E-mail: lstiburek@fld.czu.cz
article id 1674, category Research note
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković. (2016). Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.). Silva Fennica vol. 50 no. 5 article id 1674. https://doi.org/10.14214/sf.1674
Keywords: SSR markers; nuclear microsatellites; Juglans regia L.; multiplex PCR
Highlights: We combined eleven SSR markers in one multiplex PCR to make faster and cost effective amplification of the common walnut DNA from Croatia; Genetic variation of common walnut from Croatia was moderate at analyzed SSR loci; The resultant multiplex PCR could be used for genotyping of common walnut populations.
Abstract | Full text in HTML | Full text in PDF | Author Info

Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.

  • Ćelepirović, Division of Genetics, Forest Tree Breeding and Seed Science, Croatian Forest Research Institute, Jastrebarsko, Croatia E-mail: celepirovic.nevenka@gmail.com (email)
  • Karija Vlahović, DNA Laboratory, Department of Forensic Medicine&Criminology, School of Medicine, University of Zagreb, Zagreb, Croatia E-mail: monika.karija.vlahovic@mef.hr
  • Dounavi, Department of Forest Protection, Forest Research Insitute of Baden-Württemberg, Wonnhaldesttr. 4, 79100 Freiburg, Germany E-mail: aikaterini.dounavi@forst.bwl.de
  • Ivanković, Division of Genetics, Forest Tree Breeding and Seed Science, Croatian Forest Research Institute, Jastrebarsko, Croatia E-mail: mladeni@sumins.hr
article id 1415, category Research note
Zorica Šarac, Tanja Dodoš, Nemanja Rajčević, Srdjan Bojović, Petar Marin, Jelena Aleksić. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. Silva Fennica vol. 49 no. 5 article id 1415. https://doi.org/10.14214/sf.1415
Keywords: genetic differentiation; genetic diversity; the Balkans; European black pine; chloroplast microsatellites; mitochondrial nad7 intron 1; haplotype network
Highlights: Seven populations of Pinus nigra from the central Balkans, representing four infraspecific taxa, were analyzed with chloroplast microsatellites and a mitochondrial locus; Molecular data failed to support infraspecific circumscriptions; Levels of genetic diversities/differentiation at both genomes were in the range of those reported in western Mediterranean populations of P. nigra; Iberian/African and Balkans’ populations share one mtDNA polymorphism and differ in three mutations.
Abstract | Full text in HTML | Full text in PDF | Author Info

Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Đorđević; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is – exceptionally high levels of genetic diversity (HT = 0.949) and low genetic differentiation (GST = 0.024) at the cpDNA level, and moderate levels of genetic diversity (HT = 0.357) and genetic differentiation (GST = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans’ and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.

  • Šarac, University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Višegradska 33, 18000 Niš, Serbia E-mail: saraczorica@gmail.com (email)
  • Dodoš, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: tanjadodos@bio.bg.ac.rs
  • Rajčević, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: nemanja@bio.bg.ac.rs
  • Bojović, University of Belgrade, Institute for Biological Research “Siniša Stanković”, Boulevard Despota Stefana 142, 11060 Belgrade, Serbia E-mail: bojovic@ibiss.bg.ac.rs
  • Marin, University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 16, 11000 Belgrade, Serbia E-mail: pdmarin@bio.bg.ac.rs
  • Aleksić, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11000 Belgrade, Serbia E-mail: aleksic_jelena@yahoo.com.au
article id 569, category Research note
Heikki Kauhanen. (2002). Occurrence of fires in the eastern Saariselkä area, north-west Russia. Silva Fennica vol. 36 no. 1 article id 569. https://doi.org/10.14214/sf.569
Keywords: fire; anthropogenic fire; burn; Russia; satellite image; vegetation mosaic
Abstract | View details | Full text in PDF | Author Info
The occurrence of fires was studied in the eastern Saariselkä area, North-West Russia, by using satellite images and topographical maps. In total, more than 330 burned areas were pinpointed in the study area of 4770 km2. Old burns were concentrated in the eastern part of the study area, but young burns were more common in the west. Sites affected by fires in the more recent past were much smaller than those burnt over earlier. The abundance of burns along rivers and the border surveillance road provided evidence of human impact. The most significant changes in the landscape were found in the eastern part of the study area, where spruce forests had been replaced by birch woodlands.
  • Kauhanen, Finnish Forest Research Institute, Kolari Research Station, Muoniontie 21 A, FIN-95900 Kolari, Finland E-mail: heikki.kauhanen@metla.fi (email)

Category : Discussion article

article id 610, category Discussion article
Risto Päivinen, Perttu Anttila. (2001). How reliable is a satellite forest inventory? Silva Fennica vol. 35 no. 1 article id 610. https://doi.org/10.14214/sf.610
Keywords: forest inventory; remote sensing; satellite images
View details | Full text in PDF | Author Info
  • Päivinen, European Forest Institute, Torikatu 34, FIN-80101 Joensuu, Finland E-mail: risto.paivinen@efi.fi (email)
  • Anttila, European Forest Institute, Torikatu 34, FIN-80101 Joensuu, Finland E-mail: pa@nn.fi

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